Open AccessThe calcium‐dependent protein kinase (CDPK) and CDPK‐related kinase gene families in Hevea brasiliensis —comparison with five other plant species in structure, evolution, and expression
Author(s) -
Xiao XiaoHu,
Yang Meng,
Sui JinLei,
Qi JiYan,
Fang YongJun,
Hu SongNian,
Tang ChaoRong
Publication year - 2017
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12163
Subject(s) - adapter molecule crk , hevea brasiliensis , biology , gene , gene family , arabidopsis thaliana , genetics , gene expression , arabidopsis , signal transducing adaptor protein , chemistry , natural rubber , organic chemistry , mutant
Calcium‐dependent protein kinases (CDPKs or CPKs) play important roles in various physiological processes of plants, including growth and development, stress responses and hormone signaling. Although the CDPK gene family has been characterized in several model plants, little is known about this gene family in Hevea brasiliensis (the Para rubber tree). Here, we characterize the entire H. brasiliensis CDPK and CDPK‐related kinase (CRK) gene families comprising 30 CDPK genes ( HbCPK1 to 30 ) and nine CRK genes ( HbCRK1 to 9 ). Structure and phylogeny analyses of these CDPK and CRK genes demonstrate evolutionary conservation in these gene families across H. brasiliensis and other plant species. The expression of HbCPK and HbCRK genes was investigated via Solexa sequencing in a range of experimental conditions (different tissues, phases of leaf development, ethylene treatment, and various abiotic stresses). The results suggest that HbCPK and HbCRK genes are important components in growth, development, and stress responses of H. brasiliensis . Parallel studies on the CDPK and CRK gene families were also extended to five other plant species ( Arabidopsis thaliana , Oryza sativa , Populus trichocarpa , Manihot esculenta, and Ricinus communis ). The CDPK and CRK genes from different plant species that exhibit similar expression patterns tend to cluster together, suggesting a coevolution of gene structure and expression behavior in higher plants. The results serve as a foundation to further functional studies of these gene families in H. brasiliensis as well as in the whole plant kingdom.