Open AccessEukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer
Author(s) -
Lin Yaying,
Zhang Rongrong,
Zhang Ping
Publication year - 2016
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12137
Subject(s) - ovarian cancer , gene knockdown , cancer research , biology , cell cycle , cancer , gene silencing , cell growth , protein subunit , cell culture , genetics , gene
Ovarian cancer is the most common cause of gynaecological cancer‐associated death; thus, promising biomarkers and new therapeutic targets for ovarian cancer must be explored. Here, we report that eukaryotic translation initiation factor 3 subunit D (EIF3D), a member of the EIF3 family, was overexpressed in ovarian cancer clinical tissues. Furthermore, the expression of EIF3D was correlated with the International Federation of Gynecology and Obstetrics stage and pathological differentiation stage. 3‐(4,5‐dimethylthylthiazol‐2‐yl)‐2 (MTT) and colony formation assays revealed that the lentivirus‐mediated knockdown of EIF3D suppresses cell proliferation in the ovarian tumour cell lines CAOV‐3 and SKOV‐3. Flow cytometry revealed that cells were arrested at the G2/M phase of the cell cycle and that cyclin‐dependent kinase 1 was also altered after EIF3D silencing. The results presented here demonstrate that EIF3D may play an important role in the occurrence and development of ovarian cancer.