Open AccessRrp15 affects cell cycle, proliferation, and apoptosis in NIH 3T3 cells
Author(s) -
Wu Tao,
Ren MeiXia,
Chen Guoping,
Jin Zhengming,
Wang Gang
Publication year - 2016
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12128
Subject(s) - gene knockdown , microbiology and biotechnology , 3t3 cells , cell growth , apoptosis , cell cycle , biology , rna , gene , flow cytometry , nucleolus , nucleus , transfection , genetics
Riken 2810430M08 (hereinafter referred to as Rrp15) is a newly identified and reported gene from the mouse genome. In our previous work, we found that the gene had a relationship with the proliferation and activation of T cells. Rrp15 protein is highly homologous with RRP 15 (budding yeast), which has an important role in ribosomal RNA processing. We explored the potential function of Rrp15 in apoptosis, cell proliferation, and its involvement with RNA in the nucleus. We constructed a knockdown of the Rrp15 gene in NIH 3T3 cells and then performed real‐time PCR , western blotting, flow cytometry, and immunofluorescence to determine the function of the Rrp15 gene. Knockdown of the Rrp15 gene suppresses proliferation and induces apoptosis. We also found that the Rrp15 protein was normally distributed in the nucleus and bound to RNA or pre‐ RNA in the nucleus. Additionally, Rrp15 altered the activity of the 20S proteasome. Rrp15 promotes proliferation and inhibits apoptosis in NIH 3T3 cells and may have a relationship with RNA in the nucleus.