Open AccessPre‐ mRNA splicing is modulated by antifungal drugs in the filamentous fungus Neurospora crassa
Author(s) -
Mendes Niege S.,
Silva Patricia M.,
SilvaRocha Rafael,
MartinezRossi Nilce M.,
Rossi Antonio
Publication year - 2016
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12047
Subject(s) - neurospora crassa , filamentous fungus , fungus , antifungal , rna splicing , biology , crassa , microbiology and biotechnology , gene , biochemistry , botany , rna , mutant
For this study, we sought to identify pre‐ mRNA processing events modulated by changes in extracellular pH , inorganic phosphate, and antifungal drugs. We examined genes with at least four putative introns whose transcriptional level responded to these effectors. We showed that the intron retention levels of genes encoding asparagine synthetase 2, C6‐zinc finger regulator ( fluffy ), and a farnesyltransferase respond to amphotericin B, ketoconazole, and other effectors. In general, the assayed antifungals promoted the disruption of the structural domains of these proteins probably leading to their inactivation, which emphasize the complexity of the metabolic modulation exerted by antifungal signaling.