Open Accessmi RNA expression profiling of Epstein–Barr virus‐associated NKTL cell lines by Illumina deep sequencing
Author(s) -
Alles Julia,
Menegatti Jennifer,
Motsch Natalie,
Hart Martin,
Eichner Norbert,
Reinhardt Richard,
Meister Gunter,
Grässer Friedrich A.
Publication year - 2016
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12027
Subject(s) - rna , biology , microbiology and biotechnology , epstein–barr virus , gene expression , virus , non coding rna , cell culture , gene , gene expression profiling , virology , cancer research , genetics
The aim of this work was to establish the micro RNA profile of SNK 6 and SNT 16, two Epstein–Barr virus ( EBV )‐infected cell lines derived from nasal NK /T‐cell lymphoma ( NKTL ). The oncogenic EBV is strongly associated with the pathogenesis of nasal and extranodal NK /T‐cell lymphoma and expresses 44 mature micro RNA s and two noncoding EBV ‐encoded RNA s ( EBER s). mi RNA s are 19‐25nt noncoding RNA s that affect host and viral gene expression post‐transcriptionally. Deregulated mi RNA patterns are frequently linked to a variety of human cancers including lymphomas. mi RNA profiling of the two NK /T cell lines vs. primary cells revealed 10 and 4 up‐regulated and 10 and 12 down‐regulated mi RNA s in SNK 6 and SNT 16 cells respectively. The results were validated by qRT ‐ PCR for selected mi RNA s. Target gene analyses confirmed cullin 5 ( CUL 5) and sphingosin‐1‐phosphate receptor 1 (S1 PR 1) as targets for the down‐regulated hsa‐miR‐148a and viral ebv‐miR‐ BART 16 respectively. As recently demonstrated for the regulation of IL 1‐alpha by miR‐142‐3p, coexpression of the EBER s selectively exerted corepression of S1 PR 1 by BART 16 but not of CUL 5 by miR‐148a, indicating selective corepression by the EBER s.