Open AccessGerm cell‐specific expression of Cre recombinase using the VASA promoter in the pig
Author(s) -
Song Yuning,
Lai Liangxue,
Li Li,
Huang Yongye,
Wang Anfeng,
Tang Xiaochun,
Pang Daxin,
Li Zhanjun,
Ouyang Hongsheng
Publication year - 2016
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12005
Subject(s) - cre recombinase , biology , somatic cell , transgene , germ cell , recombinase , genetically modified mouse , somatic cell nuclear transfer , microbiology and biotechnology , gene , embryo , genetics , embryogenesis , blastocyst , recombination
The Cre– lox P system is a powerful tool for genetic analysis of distinct cell lineages and tissue‐specific gene knockout in animal models. VASA is specifically expressed in reproductive tissues, and is known to play important roles in spermatogenesis and germ‐cell growth. In this study, Cre recombinase transgenic pigs under the control of the VASA promoter were generated by somatic cell nuclear transfer. Germ cell‐specific expression of Cre recombinase in VASA ‐Cre transgenic pigs was shown by western blotting and immunohistochemistry. VASA ‐Cre transgenic pigs will be a useful tool for germ cell‐specific gene knockout and a disease model for disorders of the reproductive system.