z-logo
Premium
Shift of anammox bacterial community structure along the P earl E stuary and the impact of environmental factors
Author(s) -
Fu Bingbing,
Liu Jiwen,
Yang Hongmei,
Hsu Ting Chang,
He Biyan,
Dai Minhan,
Kao Shuh Ji,
Zhao Meixun,
Zhang XiaoHua
Publication year - 2015
Publication title -
journal of geophysical research: oceans
Language(s) - English
Resource type - Journals
eISSN - 2169-9291
pISSN - 2169-9275
DOI - 10.1002/2014jc010554
Subject(s) - anammox , candidatus , estuary , gammaproteobacteria , biology , anoxic waters , bacteria , salinity , environmental chemistry , 16s ribosomal rna , archaea , surface water , botany , ecology , denitrification , chemistry , nitrogen , environmental science , denitrifying bacteria , environmental engineering , organic chemistry , genetics
Anaerobic ammonium oxidation (anammox) plays an important role in the marine nitrogen cycle. The Pearl Estuary, a typical subtropical estuary characterized by hypoxia upstream and high loads of organic matter and inorganic nutrients caused by anthropogenic activities, has received extensive attention. In this study, anammox bacterial community structures in surface sediments along the Pearl Estuary were investigated using 16S rRNA and hydrazine oxidoreductase (HZO) genes. In addition, abundance of anammox bacteria in both water and surface sediments was investigated by quantitative PCR. Obvious anammox bacterial community structure shift was observed in surface sediments, in which the dominant genus changed from “ Candidatus Brocadia” or “ Candidatus Anammoxoglobus” to “ Candidatus Scalindua” along the salinity gradient from freshwater to the open ocean based on 16S rRNA gene and HZO amino acid phylotypes. This distribution pattern was associated with salinity, temperature, pH of overlying water, and particularly C/N ratio. Phylogenetic analysis unraveled a rich diversity of anammox bacteria including four novel clusters provisionally named “ Candidatus Jugangensis,” “ Candidatus Oceanicum,” “ Candidatus Anammoxidans,” and “ Candidatus Aestuarianus.” The abundance of anammox bacteria in surface sediments, bottom and surface waters ranged from 4.22 × 10 5 to 2.55 × 10 6 copies g −1 , 1.24 × 10 4 to 1.01×10 5 copies L −1 , and 8.07×10 3 to 8.86×10 5 copies L −1 , respectively. The abundance of anammox bacteria in the water column was positively correlated withNO 2 −andNO 3 − , and negatively correlated with dissolved oxygen, although an autochthonous source might contribute to the observed abundance of anammox bacteria.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here