Open AccessImplementation of the plasma MYCN / NAGK ratio to detect MYCN amplification in patients with neuroblastoma
Author(s) -
Su Yan,
Wang Lijun,
Zhao Qian,
Yue Zhixia,
Zhao Wen,
Wang Xisi,
Duan Chao,
Jin Mei,
Zhang Dawei,
Chen Shenglan,
Yin Jianfeng,
Qiu Lihua,
Cheng Xianfeng,
Xu Zhong,
Ma Xiaoli
Publication year - 2020
Publication title -
molecular oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.332
H-Index - 88
eISSN - 1878-0261
pISSN - 1574-7891
DOI - 10.1002/1878-0261.12794
Subject(s) - neuroblastoma , gene duplication , n myc , cancer research , medicine , biology , gene , genetics , cell culture , ganglioneuroma
Detection of amplification of the MYCN gene is essential for estimating treatment and prognosis of patients with neuroblastoma. We developed a method to detect MYCN amplification using the plasma DNA by quantitative PCR. An increase in MYCN copies in the plasma was consistent with MYCN amplification as assessed by DNA‐FISH to measure MYCN amplification in patients with neuroblastoma.