Open AccessThe potential of combined mutation sequencing of plasma circulating cell‐free DNA and matched white blood cells for treatment response prediction
Author(s) -
Leest Paul,
Schuuring Ed
Publication year - 2020
Publication title -
molecular oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.332
H-Index - 88
eISSN - 1878-0261
pISSN - 1574-7891
DOI - 10.1002/1878-0261.12646
Subject(s) - biology , germline mutation , dna sequencing , germline , cell free fetal dna , mutation , computational biology , single cell sequencing , haematopoiesis , genetics , dna , exome sequencing , stem cell , gene , fetus , prenatal diagnosis , pregnancy
Highly sensitive mutation detection methods enable the application of circulating cell-free DNA for molecular tumor profiling. Recent studies revealed that sequencing artifacts, germline variants, and clonal hematopoiesis confound the interpretation of sequencing results and complicate subsequent treatment decision making and disease monitoring. Parallel sequencing of matched white blood cells promises to overcome these issues and enables appropriate variant calling. Comment on: https://doi.org/10.1002/1878-0261.12617.