Open AccessNegative regulation of miR‐1275 by H3K27me3 is critical for glial induction of glioblastoma cells
Author(s) -
Mai Jialuo,
Gu Jiayu,
Liu Ying,
Liu Xincheng,
Sai Ke,
Chen Zhijie,
Lu Wanjun,
Yang Xiaozhi,
Wang Jingyi,
Guo Cui,
Sun Shuxin,
Xing Fan,
Sheng Longxiang,
Lu Bingzheng,
Zhu Zhu,
Sun Hongjiaqi,
Xue Dongdong,
Lin Yuan,
Cai Jing,
Tan Yaqian,
Li Chuntao,
Yin Wei,
Cao Lin,
Ouyang Ying,
Qiu Pengxin,
Su Xingwen,
Yan Guangmei,
Liang Jiankai,
Zhu Wenbo
Publication year - 2019
Publication title -
molecular oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.332
H-Index - 88
eISSN - 1878-0261
pISSN - 1574-7891
DOI - 10.1002/1878-0261.12525
Subject(s) - prc2 , glial fibrillary acidic protein , biology , cyclic adenosine monophosphate , microbiology and biotechnology , cellular differentiation , cancer research , protein kinase a , signal transduction , downregulation and upregulation , microrna , histone h3 , epigenetics , kinase , biochemistry , immunology , gene , receptor , immunohistochemistry
Activation of the cyclic adenosine monophosphate/protein kinase A (cAMP/PKA) pathway induces glial differentiation of glioblastoma (GBM) cells, but the mechanism by which microRNA (miRNA) regulate this process remains poorly understood. In this study, by performing miRNA genomics and loss‐ and gain‐of‐function assays in dibutyryl‐cAMP‐treated GBM cells, we identified a critical negative regulator, hsa‐miR‐1275, that modulates a set of genes involved in cancer progression, stem cell maintenance, and cell maturation and differentiation. Additionally, we confirmed that miR‐1275 directly and negatively regulates the protein expression of glial fibrillary acidic protein (GFAP), a marker of mature astrocytes. Of note, tri‐methyl‐histone H3 (Lys27) (H3K27me3), downstream of the PKA/polycomb repressive complex 2 (PRC2) pathway, accounts for the downregulation of miR‐1275. Furthermore, decreased miR‐1275 expression and induction of GFAP expression were also observed in dibutyryl‐cAMP‐treated primary cultured GBM cells. In a patient‐derived glioma stem cell tumor model, a cAMP elevator and an inhibitor of H3K27me3 methyltransferase inhibited tumor growth, induced differentiation, and reduced expression of miR‐1275. In summary, our study shows that epigenetic inhibition of miR‐1275 by the cAMP/PKA/PRC2/H3K27me3 pathway mediates glial induction of GBM cells, providing a new mechanism and novel targets for differentiation‐inducing therapy.