Open AccessExpression of long non‐coding RNA ENSG 00000226738 (Lnc KLHDC 7B) is enriched in the immunomodulatory triple‐negative breast cancer subtype and its alteration promotes cell migration, invasion, and resistance to cell death
Author(s) -
BeltránAnaya Fredy Omar,
RomeroCórdoba Sandra,
RebollarVega Rosa,
Arrieta Oscar,
BautistaPiña Verónica,
DominguezReyes Carlos,
VillegasCarlos Felipe,
TenorioTorres Alberto,
AlfaroRiuz Luis,
JiménezMorales Silvia,
CedroTanda Alberto,
RíosRomero Magdalena,
ReyesGrajeda Juan Pablo,
Tagliabue Elda,
Iorio Marilena V.,
HidalgoMiranda Alfredo
Publication year - 2019
Publication title -
molecular oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.332
H-Index - 88
eISSN - 1878-0261
pISSN - 1574-7891
DOI - 10.1002/1878-0261.12446
Subject(s) - triple negative breast cancer , gene knockdown , biology , long non coding rna , cancer research , breast cancer , microrna , rna , gene expression , non coding rna , regulation of gene expression , rna binding protein , cancer , gene , genetics
Triple negative breast cancer ( TNBC ) represents an aggressive phenotype with poor prognosis compared with ER , PR, and HER 2‐positive tumors. TNBC is a heterogeneous disease, and gene expression analysis has identified seven molecular subtypes. Accumulating evidence demonstrates that long non‐coding RNA (lnc RNA ) are involved in regulation of gene expression and cancer biology, contributing to essential cancer cell functions. In this study, we analyzed the expression profile of lnc RNA in TNBC subtypes from 156 TNBC samples, and then characterized the functional role of Lnc KLHDC 7B ( ENSG 226738 ). A total of 710 lnc RNA were found to be differentially expressed between TNBC subtypes, and a subset of these altered lnc RNA were independently validated. We discovered that Lnc KLHDC 7B ( ENSG 226738 ) acts as a transcriptional modulator of its neighboring coding gene KLHDC 7B in the immunomodulatory subtype. Furthermore, Lnc KLHDC 7B knockdown enhanced migration and invasion, and promoted resistance to cellular death. Our findings confirmed the contribution of Lnc KLHDC 7B to induction of apoptosis and inhibition of cell migration and invasion, suggesting that TNBC tumors with enrichment of Lnc KLHDC 7B may exhibit distinct regulatory activity, or that this may be a generalized process in breast cancer. Additionally, in silico analysis confirmed for the first time that the low expression of KLHDC 7B and Lnc KLHDC 7B is associated with poor prognosis in patients with breast cancer.