Open AccessHypoxia‐sensitive LINC 01436 is regulated by E2F6 and acts as an oncogene by targeting miR‐30a‐3p in non‐small cell lung cancer
Author(s) -
Yuan Shuai,
Xiang Ying,
Wang Guilu,
Zhou Meiyu,
Meng Gang,
Liu Qingyun,
Hu Zeyao,
Li Chengying,
Xie Weijia,
Wu Na,
Wu Long,
Cai Tongjian,
Ma Xiangyu,
Zhang Yao,
Yu Zubin,
Bai Li,
Li Yafei
Publication year - 2019
Publication title -
molecular oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.332
H-Index - 88
eISSN - 1878-0261
pISSN - 1574-7891
DOI - 10.1002/1878-0261.12437
Subject(s) - microrna , biology , oncogene , cancer research , long non coding rna , lung cancer , rna , metastasis , cancer , cell growth , gene , cell cycle , medicine , pathology , genetics
Dysregulation of long noncoding RNA (lncRNA) is known to be involved in numerous human diseases, including lung cancer. However, the precise biological functions of most lnc RNA remain to be elucidated. Here, we identified a novel up‐regulated lnc RNA , LINC 01436 (RefSeq: NR _110419.1), in non‐small cell lung cancer ( NSCLC ). High expression of LINC 01436 was significantly associated with poor overall survival. Notably, LINC 01436 expression was transcriptionally repressed by E2F6 under normoxia, and the inhibitory effect was relieved in a hypoxic microenvironment. Gain‐ and loss‐of‐function studies revealed that LINC 01436 acted as a proto‐oncogene by promoting lung cancer cell growth, migration and invasion in vitro . Xenograft tumor assays in nude mice confirmed that LINC 01436 promoted tumor growth and metastasis in vivo . Mechanistically, LINC 01436 exerted biological functions by acting as a microRNA (miR)‐30a‐3p sponge to regulate the expression of its target gene EPAS 1 . Our findings characterize LINC 01436 as a new hypoxia‐sensitive lnc RNA with oncogenic function in NSCLC , suggesting that LINC 01436 may be a potential biomarker for prognosis and a potential target for treatment.