Open AccessCircular RNA profiling identifies circ ADAMTS 13 as a miR‐484 sponge which suppresses cell proliferation in hepatocellular carcinoma
Author(s) -
Qiu Liman,
Huang Yanbing,
Li Zhenli,
Dong Xiuqing,
Chen Geng,
Xu Haipo,
Zeng Yongyi,
Cai Zhixiong,
Liu Xiaolong,
Liu Jingfeng
Publication year - 2019
Publication title -
molecular oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.332
H-Index - 88
eISSN - 1878-0261
pISSN - 1574-7891
DOI - 10.1002/1878-0261.12424
Subject(s) - circular rna , carcinogenesis , microrna , cancer research , hepatocellular carcinoma , biology , tumor progression , cell growth , microbiology and biotechnology , gene , genetics
Circular RNA (circ RNA ) can participate in various biological processes, including tumorigenesis, through their micro RNA response elements. Alterations in circ RNA profiles during hepatocellular carcinoma ( HCC ) progression and their clinical significance remain unclear. Here, we present extensive analysis of circ RNA profiles in tumor and matched peritumor tissues collected from 10 HCC patients, conducted to identify circ RNA related to HCC progression. A total of 42 dysregulated circ RNA (38 down‐regulated and 4 up‐regulated) were identified in HCC tumor tissues compared with matched peritumor tissues, revealing the heterogeneity of circ RNA profiles in HCC . Circ ADAMTS 13, derived from Exon 13–14 of the ADAMTS 13 gene, was significantly downregulated in HCC tumor tissues. Furthermore, clinicopathological analysis revealed that up‐regulation of circ ADAMTS 13 was negatively associated with tumor size but positively associated with prognosis. In addition, overexpression of circ ADAMTS 13 could markedly inhibit HCC cell proliferation in vitro . Bioinformatic analysis and luciferase reporter assays further revealed that circ ADAMTS 13 directly interacts with microRNA (miR)‐484. Rescue experiments showed that miR‐484 mimics can reverse the tumor‐suppressing roles of circ ADAMTS 13 in HCC . Therefore, our results demonstrated that circ ADAMTS 13 can serve as a tumor suppressor during HCC progression via the functional pathway of sponging miR‐484.