Open AccessExosomal micro RNA s as tumor markers in epithelial ovarian cancer
Author(s) -
Pan Chi,
Stevic Ines,
Müller Volkmar,
Ni Qingtao,
OliveiraFerrer Leticia,
Pantel Klaus,
Schwarzenbach Heidi
Publication year - 2018
Publication title -
molecular oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.332
H-Index - 88
eISSN - 1878-0261
pISSN - 1574-7891
DOI - 10.1002/1878-0261.12371
Subject(s) - microvesicles , microrna , ovarian cancer , taqman , rna , exosome , cancer research , medicine , cystadenoma , apoptosis , circular rna , cancer , biology , real time polymerase chain reaction , gene , pancreas , biochemistry
Specific micro RNA s (mi RNA s) are packaged in exosomes that regulate processes in tumor development and progression. The current study focuses on the influence of exosomal mi RNA s in the pathogenesis of epithelial ovarian cancer ( EOC ). Mi RNA profiles were determined in exosomes from plasma of 106 EOC patients, eight ovarian cystadenoma patients, and 29 healthy women by TaqMan real‐time PCR ‐based mi RNA array cards containing 48 different mi RNA s. In cell culture experiments, the impact of miR‐200b and miR‐320 was determined on proliferation and apoptosis of ovarian cancer cell lines. We report that miR‐21 ( P = 0.0001), miR‐100 ( P = 0.034), miR‐200b ( P = 0.008), and miR‐320 ( P = 0.034) are significantly enriched, whereas miR‐16 ( P = 0.009), miR‐93 ( P = 0.014), miR‐126 ( P = 0.012), and miR‐223 ( P = 0.029) are underrepresented in exosomes from plasma of EOC patients as compared to those of healthy women. The levels of exosomal miR‐23a ( P = 0.009, 0.008) and miR‐92a ( P = 009, 0.034) were lower in ovarian cystadenoma patients than in EOC patients and healthy women, respectively. The exosomal levels of miR‐200b correlated with the tumor marker CA 125 ( P = 0.002) and patient overall survival ( P = 0.019). MiR‐200b influenced cell proliferation ( P = 0.0001) and apoptosis ( P < 0.008). Our findings reveal specific exosomal mi RNA patterns in EOC and ovarian cystadenoma patients, which are indicative of a role of these mi RNAs in the pathogenesis of EOC .