Open AccessAberrant expression of kallikrein‐related peptidase 7 is correlated with human melanoma aggressiveness by stimulating cell migration and invasion
Author(s) -
Delaunay Tiphaine,
Deschamps Lydia,
Haddada Meriem,
Walker Francine,
Soosaipillai Antoninus,
Soualmia Feryel,
El Amri Chahrazade,
Diamandis Eleftherios P.,
Brattsand Maria,
Magdolen Viktor,
Darmoul Dalila
Publication year - 2017
Publication title -
molecular oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.332
H-Index - 88
eISSN - 1878-0261
pISSN - 1574-7891
DOI - 10.1002/1878-0261.12103
Subject(s) - melanoma , cancer research , biology , biomarker , ectopic expression , immunohistochemistry , cancer , phenotype , tumor progression , metastasis , cell , cell culture , pathology , immunology , medicine , gene , genetics
Members of the tissue kallikrein‐related peptidase ( KLK ) family not only regulate several important physiological functions, but aberrant expression has also been associated with various malignancies. Clinically, KLK s have been suggested as promising biomarkers for diagnosis and prognosis in many types of cancer. As of yet, expression of KLK s and their role in skin cancers are, however, poorly addressed. Malignant melanoma is an aggressive disease associated with poor prognosis. Hence, diagnostic biomarkers to monitor melanoma progression are needed. Herein, we demonstrate that although mRNA of several KLK s are aberrantly expressed in melanoma cell lines, only the KLK 7 protein is highly secreted in vitro . In line with these findings, ectopic expression of KLK 7 in human melanomas and its absence in benign nevi were demonstrated by immunohistochemistry in vivo . Interestingly, overexpression of KLK 7 induced a significant reduction in melanoma cell proliferation and colony formation. Moreover, KLK 7 overexpression triggered an increase in cell motility and invasion associated with decreased expression of E‐cadherin and an upregulation of MCAM / CD 146. Our results demonstrate, for the first time, that aberrant KLK 7 expression leads to a switch from proliferative to invasive phenotype, suggesting a potential role of KLK 7 in melanoma progression. Thus, we hypothesize that KLK 7 may represent a potential biomarker for melanoma progression.