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EZH2 modulates retinoic acid signaling to ensure myotube formation during development
Author(s) -
Thulabandu Venkata,
Ferguson James W.,
Phung Melissa,
Atit Radhika P.
Publication year - 2022
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.14334
Subject(s) - myogenesis , prc2 , retinoic acid , ezh2 , myocyte , microbiology and biotechnology , cellular differentiation , signal transduction , biology , progenitor cell , chemistry , stem cell , biochemistry , genetics , cell culture , epigenetics , gene
Sequential differentiation of presomitic progenitors into myocytes and subsequently into myotubes and myofibers is essential for the myogenic differentiation program (MDP) crucial for muscle development. Signaling factors involved in MDP are polycomb repressive complex 2 (PRC2) targets in various developmental contexts. PRC2 is active in the developing myotomes during MDP, but how it regulates MDP is unclear. Here, we found that myocyte differentiation to myotubes requires Enhancer of Zeste 2 (EZH2), the catalytic component of PRC2. We observed elevated retinoic acid (RA) signaling in the prospective myocytes in the Ezh2 mutants (E8.5‐Mus Ezh2 ), and its inhibition can partially rescue the myocyte differentiation defect. Together, our data demonstrate a new role for PRC2–EZH2 during myocyte differentiation into myotubes by modulating RA signaling.