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α‐Syntrophin alleviates ER stress to maintain protein homeostasis during myoblast differentiation
Author(s) -
Moon Jae Yun,
Kim Hye Sun
Publication year - 2021
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.14088
Subject(s) - endoplasmic reticulum , microbiology and biotechnology , unfolded protein response , myocyte , intracellular , chemistry , cellular differentiation , biology , biochemistry , gene
We have previously shown evidence that α‐syntrophin plays an important role in myoblast differentiation. In this study, we focused on abnormal myotube formation of the α‐syntrophin knockdown C2 cell line (SNKD). The overall amount of intracellular protein and muscle‐specific proteins in SNKD cells were significantly lower than those in the control. Akt‐mTOR signaling, an important pathway for protein synthesis and muscle hypertrophy, was downregulated. In addition, the levels of endoplasmic reticulum (ER) stress markers increased in SNKD cells. The decrease in intracellular protein synthesis and reduction in the myotube diameter in SNKD cells were restored by 4‐phenylbutyric acid, a chemical chaperone, or overexpression of α‐syntrophin. These results suggest a novel role for α‐syntrophin in protein homeostasis during myoblast differentiation.