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Generation and characterization of a high‐affinity chimeric anti‐OX40 antibody with potent antitumor activity
Author(s) -
Yang Yongli,
Chai Xiaojuan,
Xin Wenfang,
Wang Dongxu,
Dai Chaohui,
Qian Feng,
Yang Teddy
Publication year - 2021
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.14079
Subject(s) - monoclonal antibody , antibody , epitope , immunization , cancer immunotherapy , immunotherapy , fusion protein , antigen , immune system , recombinant dna , cancer research , immunology , microbiology and biotechnology , biology , chemistry , biochemistry , gene
OX40 is a costimulatory molecule that belongs to the tumor necrosis factor receptor (TNFR) superfamily. OX40 agonist‐based combinations are emerging as promising candidates for novel cancer immunotherapy. Clinical trials have shown that OX40 agonist antibodies could lead to better results in cancer patients. Using a hybridoma platform and three different types of immunization strategies, namely recombinant protein, DNA, and overexpressing cells, we identified a chimeric anti‐OX40 antibody (mAb035‐hIgG1 from DNA immunization) that shows excellent binding specificity, and slightly stronger activation of human memory CD4 + T cells and similar potent antitumor activity compared with BMS 986178, an anti‐OX40 antibody currently being evaluated for the treatment of solid tumors. This paper further systematically investigates the antigen‐specific immune response, the number of binders, epitope bins, and functional activities of antibodies among different immunization strategies. Interestingly, we found that different immunization strategies affect the biological activity of monoclonal antibodies.