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Centrosomal protein FOR20 knockout mice display embryonic lethality and left‐right patterning defects
Author(s) -
Xu Zhangqi,
Liu Min,
Gao Cheng,
Kuang Wenjun,
Chen Xiying,
Liu Feifei,
Ge Bai,
Yan Xiaoyi,
Zhou Tianhua,
Xie Shanshan
Publication year - 2021
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.14071
Subject(s) - embryonic stem cell , biology , ciliogenesis , microbiology and biotechnology , knockout mouse , gene knockout , embryogenesis , conditional gene knockout , embryo , gene targeting , cilium , heterozygote advantage , genetics , gene , phenotype , allele
Centrosomal protein FOR20 has been reported to be crucial for essential cellular processes, including ciliogenesis, cell migration, and cell cycle in vertebrates. However, the function of FOR20 during mammalian embryonic development remains unknown. To investigate the in vivo function of the For20 gene in mammals, we generated For20 homozygous knockout mice by gene targeting. Our data reveal that homozygous knockout of For20 results in significant embryonic growth arrest and lethality during gestation, while the heterozygotes show no obvious defects. The absence of For20 leads to impaired left‐right patterning of embryos and reduced cilia in the embryonic node. Deletion of For20 also disrupts angiogenesis in yolk sacs and embryos. These results highlight a critical role of For20 in early mammalian embryogenesis.