Premium
Human fibroblast‐derived extracellular vesicles promote hair growth in cultured human hair follicles
Author(s) -
Rajendran Ramya Lakshmi,
Gangadaran Prakash,
Kwack Mi Hee,
Oh Ji Min,
Hong Chae Moon,
Sung Young Kwan,
Lee Jaetae,
Ahn ByeongCheol
Publication year - 2021
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.14050
Subject(s) - outer root sheath , microbiology and biotechnology , hair follicle , fibroblast , cytoplasm , chemistry , extracellular , root hair , nucleus , biology , in vitro , biochemistry , gene
Hair loss is a prevalent medical condition affecting both genders. In this study, we investigate the effects of a specific class of extracellular vesicles (EVs), namely human normal fibroblast‐derived EVs (hFB‐EVs), on human dermal papilla (DP) and outer root sheath (ORS) cells and examine the molecular mechanisms responsible for hair growth in hair follicles (HFs). We find that Wnt3a, which maintains the hair‐generating activity of DP cells, is enriched and more strongly associated with hFB‐EVs than with fibroblasts. Furthermore, hFB‐EV‐associated Wnt3a mediated receptor activation in cultured DP cells, leading to an increase in β‐catenin in the cytoplasm and its translocation into the nucleus, thereby elevating expression of the target genes Axin2 and Lef1. Additionally, hFB‐EVs promoted the migration, proliferation, and differentiation of ORS cells and elongation of the hair shaft in human HFs. These findings revealed a novel mechanism by which hFB‐EVs influence hair growth.