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Subunits of the GPI transamidase complex localize to the endoplasmic reticulum and nuclear envelope in Drosophila
Author(s) -
Kawaguchi Kohei,
YamamotoHino Miki,
Matsuyama Nina,
Suzuki Emiko,
Goto Satoshi
Publication year - 2021
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.14048
Subject(s) - endoplasmic reticulum , protein subunit , microbiology and biotechnology , nucleus , drosophila (subgenus) , biology , chemistry , biochemistry , gene
A total of 10–20% of plasma membrane proteins are anchored by glycosylphosphatidylinositol (GPI). GPI is attached to proteins by GPI transamidase (GPI‐T), which contains five subunits named PIGK, PIGS, PIGT, PIGU, and GPAA1. We previously reported that PIGT localizes near the nucleus in Drosophila . However, localizations of the other four subunits remain unknown. Here, we show that a catalytic subunit of GPI‐T, PIGK, mainly localizes to the endoplasmic reticulum (ER), while the other four subunits localize to the nuclear envelope (NE) and ER. The NE/ER localization ratio of PIGS differs between cell types and developmental stages. Our results suggest that GPI‐T catalyzes GPI attachment in the ER and the other four subunits may have other unknown functions in the NE.