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The coordinated action of glucuronoyl esterase and α‐glucuronidase promotes the disassembly of lignin–carbohydrate complexes
Author(s) -
Raji Olanrewaju,
Arnling Bååth Jenny,
Vuong Thu V.,
Larsbrink Johan,
Olsson Lisbeth,
Master Emma R.
Publication year - 2021
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.14019
Subject(s) - chemistry , lignin , esterase , substrate (aquarium) , decomposition , biochemistry , biomass (ecology) , polysaccharide , carbohydrate , food science , enzyme , organic chemistry , biology , ecology , agronomy
Glucuronoxylans represent a significant fraction of woody biomass, and its decomposition is complicated by the presence of lignin–carbohydrate complexes (LCCs). Herein, LCCs from birchwood were used to investigate the potential coordinated action of a glucuronoyl esterase ( Tt CE15A) and two α‐glucuronidases ( Sde Agu115A and Axy Agu115A). When supplementing α‐glucuronidase with equimolar quantities of Tt CE15A, total MeGlc p A released after 72 h by Sde Agu115A and Axy Agu115A increased from 52% to 67%, and 61% to 95%, respectively. Based on the combined Tt CE15A and Axy Agu115A activities, ~ 34% of MeGlc p A in the extracted birchwood glucuronoxylan was occupied as LCCs. Notably, insoluble LCC fractions reduced soluble α‐glucuronidase concentrations by up to 70%, whereas reduction in soluble Tt CE15A was less than 30%, indicating different tendencies to adsorb onto the LCC substrate.