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ABRO1 stabilizes the deubiquitinase BRCC3 through inhibiting its degradation mediated by the E3 ubiquitin ligase WWP2
Author(s) -
Zhang Wen,
Tao ShouSong,
Wang Ting,
Zhang Jie,
Liu Xian,
Li YaTing,
Chen Hui,
Zhan YiQun,
Yu Miao,
Ge ChangHui,
Li ChangYan,
Ren GuangMing,
Yang XiaoMing,
Yin RongHua
Publication year - 2021
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13970
Subject(s) - deubiquitinating enzyme , ubiquitin , ubiquitin ligase , microbiology and biotechnology , proteasome , protein subunit , protein degradation , biology , chemistry , biochemistry , gene
BRCA1/BRCA2‐containing complex subunit 3 (BRCC3) is a lysine 63‐specific deubiquitinase involved in multiple biological processes, such as DNA repair and immune responses. However, the regulation mechanism for BRCC3 protein stability is still unknown. Here, we demonstrate that BRCC3 is mainly degraded through the ubiquitin‐proteasome pathway. The HECT‐type E3 ubiquitin ligase WWP2 modulates BRCC3 ubiquitination and degradation. ABRO1, a subunit of the BRCC36 isopeptidase complex (BRISC), competes with WWP2 to bind to BRCC3, thereby preventing WWP2‐mediated BRCC3 ubiquitination and enhancing BRCC3 stability. Functionally, we show that lentivirus‐mediated overexpression of WWP2 in murine macrophages inhibits NLRP3 inflammasome activation by decreasing BRCC3 protein level. This study provides the first insights into the regulation of BRCC3 stability and expands our knowledge about the physiological function of WWP2.