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Comprehensive analysis of circular RNAs from steatotic livers after ischemia and reperfusion injury by next‐generation RNA sequencing
Author(s) -
Qu Xiaoye,
Zheng Chao,
Wang Bingrui,
Wang Fang,
Sun Xuehua,
Gao Yueqiu,
Xia Qiang,
Kong Xiaoni
Publication year - 2021
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13960
Subject(s) - steatosis , circular rna , sanger sequencing , economic shortage , reperfusion injury , biology , liver transplantation , rna , ischemia , transplantation , dna sequencing , bioinformatics , gene , medicine , biochemistry , endocrinology , linguistics , philosophy , government (linguistics)
Global organ shortage has led to the acceptance of steatotic livers for transplantation, taking the risk of graft dysfunction associated with the higher sensitivity of steatotic livers to ischemia and reperfusion injury (IRI). Data about circular RNAs (circRNAs) in steatotic livers following IRI are practically nonexistent. In our study, a high‐fat diet‐fed mouse model of hepatic steatosis was generated, and RNA sequencing was performed both on IRI and on sham liver tissues of these mice to screen for circRNAs with significant differential expression. To further validate our bioinformatics data, one upregulated circRNA and four downregulated circRNAs were examined. The circularity of these circRNAs was demonstrated using RNaseR digestion and Sanger sequencing. The expression of four stable circRNAs undigested by RNaseR was further validated by quantitative PCR. In summary, this study unearths several circRNAs as novel and potentially effective targets involved in the more severe damage of steatotic livers following IRI.