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Synthetic in vitro transcribed lncRNAs (SINEUPs) with chemical modifications enhance target mRNA translation
Author(s) -
Toki Naoko,
Takahashi Hazuki,
Zucchelli Silvia,
Gustincich Stefano,
Carninci Piero
Publication year - 2020
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13928
Subject(s) - translation (biology) , messenger rna , rna , protein biosynthesis , microbiology and biotechnology , in vitro , biology , computational biology , chemistry , biochemistry , gene
Chemically modified mRNAs are extensively studied with a view toward their clinical application. In particular, long noncoding RNAs (lncRNAs) containing SINE elements, which enhance the translation of their target mRNAs (i.e., SINEUPs), have potential as RNA therapies for various diseases, such as haploinsufficiencies. To establish a SINEUP‐based system for efficient protein expression, we directly transfected chemically modified in vitro transcribed (mIVT) SINEUP RNAs to examine their effects on target mRNA translation. mIVT SINEUP RNAs enhanced translation of EGFP mRNA and endogenous target Sox9 mRNA in both cultured cells and a cell‐free translation system. Our findings reveal the functional role of RNA modifications in SINEUPs and suggest several broad clinical applications of such an RNA regulatory system.