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Cryo‐EM structure of the ribosome functional complex of the human pathogen Staphylococcus aureus at 3.2 Å resolution
Author(s) -
Golubev Alexander,
Fatkhullin Bulat,
Khusainov Iskander,
Jenner Lasse,
Gabdulkhakov Azat,
Validov Shamil,
Yusupova Gulnara,
Yusupov Marat,
Usachev Konstantin
Publication year - 2020
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13915
Subject(s) - staphylococcus aureus , ribosome , human pathogen , pathogen , rna , microbiology and biotechnology , transfer rna , antibiotics , biology , computational biology , chemistry , bacteria , genetics , gene
Staphylococcus aureus is a bacterial pathogen and one of the leading causes of healthcare‐acquired infections in the world. The growing antibiotic resistance of S. aureus obliges us to search for new drugs and treatments. As the majority of antibiotics target the ribosome, knowledge of its detailed structure is crucial for drug development. Here, we report the cryo‐EM reconstruction at 3.2 Å resolution of the S. aureus ribosome with P‐site tRNA, messenger RNA, and 10 RNA modification sites previously not assigned or visualized. The resulting model is the most precise and complete high‐resolution structure to date of the S. aureus 70S ribosome with functional ligands.