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Transcriptional shifts in delignification‐defective mutants of the white‐rot fungus Pleurotus ostreatus
Author(s) -
Wu Hongli,
Nakazawa Takehito,
Takenaka Atsuki,
Kodera Rina,
Morimoto Ryota,
Sakamoto Masahiro,
Honda Yoichi
Publication year - 2020
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13890
Subject(s) - pleurotus ostreatus , mutant , transcriptome , biology , gene , lignin , fungus , microbiology and biotechnology , pleurotus , genetics , gene expression , botany , mushroom
White‐rot fungi efficiently degrade lignin and, thus, play a pivotal role in the global carbon cycle. However, the mechanisms of lignin degradation are largely unknown. Recently, mutations in four genes, namely wtr1 , chd1 , pex1 , and gat1 , were shown to abrogate the wood lignin‐degrading ability of Pleurotus ostreatus . In this study, we conducted a comparative transcriptome analysis to identify genes that are differentially expressed in ligninolysis‐deficient mutant strains. Putative ligninolytic genes that are highly expressed in parental strains are significantly downregulated in the mutant strains. On the contrary, many putative cellulolytic and xylanolytic genes are upregulated in the chd1‐1 , Δ pex1 , and Δ gat1 strains. Identifying transcriptional alterations in mutant strains could provide new insights into the regulatory mechanisms of lignocellulolytic genes in P. ostreatus .