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Characterization and in vitro assembly of tick‐borne encephalitis virus C protein
Author(s) -
Kaufman Filip,
Dostálková Alžběta,
Pekárek Lukáš,
Thanh Tung Dinh,
Kapisheva Marina,
Hadravová Romana,
Bednárová Lucie,
Novotný Radim,
Křížová Ivana,
Černý Jiří,
Grubhoffer Libor,
Ruml Tomáš,
Hrabal Richard,
Rumlová Michaela
Publication year - 2020
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13857
Subject(s) - tick borne encephalitis virus , virology , circular dichroism , flavivirus , dengue virus , virus , encephalitis , biology , in vitro , recombinant dna , chemistry , biochemistry , gene
Tick‐borne encephalitis virus (TBEV), a member of flaviviruses, represents a serious health threat by causing human encephalitis mainly in central and eastern Europe, Russia, and northeastern Asia. As no specific therapy is available, there is an urgent need to understand all steps of the TBEV replication cycle at the molecular level. One of the critical events is the packaging of flaviviral genomic RNA by TBEV C protein to form a nucleocapsid. We purified recombinant TBEV C protein and used a combination of physical–chemical approaches, such as size‐exclusion chromatography, circular dichroism, NMR spectroscopies, and transmission electron microscopy, to analyze its structural stability and its ability to dimerize/oligomerize. We compared the ability of TBEV C protein to assemble in vitro into a nucleocapsid‐like structure with that of dengue C protein.