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Nuclear export of replication protein A in the nonreplicative infective forms of Trypanosoma cruzi
Author(s) -
Pavani Raphael S.,
Lima Loyze P.,
Lima André A.,
Fernandes Carlos A. H.,
Fragoso Stenio P.,
Calderano Simone G.,
Elias Maria Carolina
Publication year - 2020
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13755
Subject(s) - replication protein a , dna replication , trypanosoma cruzi , biology , nuclear export signal , protein subunit , microbiology and biotechnology , dna damage , dna , heterotrimeric g protein , nuclear transport , cytoplasm , dna binding protein , cell nucleus , genetics , gene , parasite hosting , signal transduction , transcription factor , g protein , world wide web , computer science
Replication protein A (RPA), a heterotrimeric complex, is the major single‐stranded DNA binding protein in eukaryotes. Recently, we characterized RPA from Trypanosoma cruzi , showing that it is involved in DNA replication and DNA damage response in this organism. Better efficiency in differentiation from epimastigote to metacyclic trypomastigote forms was observed in TcRPA‐2 subunit heterozygous knockout cells, suggesting that RPA is involved in this process. Here, we show that RPA cellular localization changes during the T. cruzi life cycle, with RPA being detected only in the cytoplasm of the metacyclic and bloodstream trypomastigotes. We also identify a nuclear export signal (NES) in the trypanosomatid RPA‐2 subunit. Mutations in the negatively charged residues of RPA‐2 NES impair the differentiation process, suggesting that RPA exportation affects parasite differentiation into infective forms.