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Does genome surveillance explain the global discrepancy between binding and effect of chromatin factors?
Author(s) -
Singh Arashdeep,
Choudhuri Poulami,
Chandradoss Keerthivasan Raanin,
Lal Mohan,
Mishra Shravan Kumar,
Sandhu Kuljeet Singh
Publication year - 2020
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13729
Subject(s) - chromatin , epigenetics , transcription factor , biology , genetics , promoter , chia pet , chromatin immunoprecipitation , computational biology , epigenesis , gene , chromatin remodeling , gene expression , dna methylation
Knocking out a chromatin factor often does not alter the transcription of its binding targets. What explains the observed disconnect between binding and effect? We hypothesize that this discrepancy could be associated with the role of chromatin factors in maintaining genetic and epigenetic integrity at promoters, and not necessarily with transcription. Through re‐analysis of published datasets, we present several lines of evidence that support our hypothesis and deflate the popular assumptions. We also tested the hypothesis through mutation accumulation assays on yeast knockouts of chromatin factors. Altogether, the proposed hypothesis presents a simple explanation for the global discord between chromatin factor binding and effect. Future work in this direction might fortify the hypothesis and elucidate the underlying mechanisms.