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Imprinted X‐chromosome inactivation impacts primitive endoderm differentiation in mouse blastocysts
Author(s) -
Fukuda Atsushi,
Motosugi Nami,
Ando Mikiko,
Kimura Minoru,
Umezawa Akihiro,
Akutsu Hidenori
Publication year - 2020
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13676
Subject(s) - epiblast , biology , transcriptome , endoderm , lineage (genetic) , inner cell mass , epigenetics , embryo , genetics , x inactivation , microbiology and biotechnology , germ layer , epigenomics , genomic imprinting , transcription factor , embryonic stem cell , dna methylation , blastocyst , embryogenesis , x chromosome , gene , gastrulation , gene expression , induced pluripotent stem cell
Epigenetic and transcriptome alterations are essential for lineage specification, represented by imprinted X‐chromosome inactivation ( iXCI ) in female mouse preimplantation embryos. However, how various factors affect transcriptome states and lineage commitment remains unclear. We found that in vitro culture duration strongly influences transcriptional variation compared to iXCI loss. Single‐cell analysis of the inner cell mass (ICM) for major transcription and epigenomic factors revealed that sex‐specific differences in expression are diminished by loss of iXCI in the primitive endoderm (PrE) but not in the epiblast. Females had a higher proportion of ICM compared to that in males, and PrE development was affected by iXCI states in female embryos. Our findings provide insight into sex differences and iXCI function in lineage specification.