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A newly identified photolyase from Arthrospira platensis possesses a unique methenyltetrahydrofolate chromophore‐binding pattern
Author(s) -
Yan Hui,
Zhu Kongfu,
Teng Maikun,
Li Xu
Publication year - 2020
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13657
Subject(s) - photolyase , pyrimidine dimer , chromophore , dna , transfer efficiency , dna repair , biology , chemistry , biochemistry , biophysics , photochemistry , biochemical engineering , engineering
Cyclobutane pyrimidine dimers (CPD), as a common DNA damage caused by UV radiation, often lead to skin cancer. Here, we identified a photolyase from the alga Arthrospira platensis (designated as Ap‐ phr), which has been regarded as a safe organism for humans for centuries, that can efficiently repair CPD lesions in ssDNA and dsDNA in vitro . The 1.6 Å resolution crystal structure of Ap‐ phr revealed that it possesses a unique methenyltetrahydrofolate chromophore‐binding pattern with high energy transfer efficiency. Our study of Ap‐ phr highlights its potential use in cosmetic, industrial and aesthetic medicine applications.