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Chlamydia trachomatis ‐infected human cells convert ceramide to sphingomyelin without sphingomyelin synthases 1 and 2
Author(s) -
Tachida Yuriko,
Kumagai Keigo,
Sakai Shota,
Ando Shuji,
Yamaji Toshiyuki,
Hanada Kentaro
Publication year - 2020
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13632
Subject(s) - ceramide , sphingomyelin , chlamydia trachomatis , endoplasmic reticulum , golgi apparatus , biology , sphingolipid , microbiology and biotechnology , biochemistry , virology , membrane , apoptosis
The obligate intracellular bacterium Chlamydia trachomatis proliferates in the membranous compartment inclusion formed in host cells. The host ceramide transport protein CERT delivers ceramide from the endoplasmic reticulum to the Golgi complex for the synthesis of sphingomyelin (SM). Chlamydia trachomatis has been suggested to employ CERT to produce SM in the inclusion by host SM synthases (SMSs). Here, we found that C. trachomatis proliferates and produces infective progeny even in SMS1 and SMS2 double‐knockout HeLa cells, but not in the SMS1 / SMS2 / CERT triple‐knockout cells. Interestingly, infected cells convert ceramide to SM without host SMSs. These results suggest that C. trachomatis ‐infected cells can convert ceramide to SM without host SMSs after CERT‐mediated transfer of ceramide to the inclusions.