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Single‐cell RNA sequencing reveals chemokine self‐feeding of myeloma cells promotes extramedullary metastasis
Author(s) -
Geng Shuang,
Wang Jing,
Zhang Xiannian,
Zhang Jiajia,
Wu Fan,
Pang Yuhong,
Zhong Yuping,
Wang Jianbin,
Wang Wenming,
Lyu Xiaoqing,
Huang Yanyi,
Jing Hongmei
Publication year - 2020
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13623
Subject(s) - chemokine , multiple myeloma , cancer research , metastasis , biology , immunology , genetics , inflammation , cancer
In this study, we aimed to determine the mechanisms underlying the initial extramedullary translocation of myeloma cells from bone marrow into peripheral blood. We found that clonal circulating plasma cells (cPCs) are more frequently detected by flow cytometry in extramedullary plasmacytoma (EMP) patients and worsen their prognosis. It is technically much easier to collect single cPCs using FACS than it is to perform EMP biopsy. Therefore, combining EMP imaging with cPC detection may be a promising strategy for prognostic stratification. Here, using single‐cell transcriptome analysis, we found that the chemokine CXCL12, a key molecule involved in CXCR4‐dependent cell retention in the bone marrow, is abnormally upregulated in cPCs and might initially enable cPCs to evade bone marrow retention and translocate into the bloodstream.