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2‐oxoglutarate modulates the affinity of FurA for the ntcA promoter in Anabaena sp. PCC 7120
Author(s) -
Guío Jorge,
SarasaBuisan Cristina,
VelázquezCampoy Adrián,
Bes María Teresa,
Fillat María F.,
Peleato María Luisa,
Sevilla Emma
Publication year - 2020
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13610
Subject(s) - anabaena , nitrogen fixation , chemistry , biochemistry , metabolite , transcriptional regulation , nitrogen , microbiology and biotechnology , transcription factor , biology , cyanobacteria , gene , genetics , bacteria , organic chemistry
2‐oxoglutarate (2‐OG) is a central metabolite that acts as a signaling molecule informing about the status of the carbon/nitrogen balance of the cell. In recent years, some transcriptional regulators and even two‐component systems have been described as 2‐OG sensors. In the nitrogen‐fixing cyanobacterium Anabaena sp. PCC 7120, two master regulators, NtcA and FurA, are deeply involved in the regulation of nitrogen metabolism. Both of them show a complex intertwined regulatory circuit to achieve a suitable regulation of nitrogen fixation. In this work, 2‐OG is found to bind FurA, modulating the specific binding of FurA to the ntcA promoter. This study provides evidence of a new additional control point in the complex network controlled by the NtcA and FurA proteins.