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Type 2 diabetes differentially affects the substrate saturation kinetic attributes of erythrocyte hexokinase and phosphofructokinase
Author(s) -
Bhise Sunita,
Rao Janhavi,
Hegde Mahabaleshwar,
Katyare Surendra
Publication year - 2020
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13604
Subject(s) - phosphofructokinase , hexokinase , enzyme kinetics , glycolysis , substrate (aquarium) , enzyme , biochemistry , chemistry , protein subunit , pyruvate kinase , kinetic energy , biophysics , metabolism , kinetics , endocrinology , biology , active site , ecology , physics , quantum mechanics , gene
The substrate kinetic parameters of hexokinase (HK) and phosphofructokinase (PFK)—the key irreversible enzymes of glycolysis—in erythrocytes from type 2 diabetic subjects were examined in comparison with control subjects. It was observed that the kinetic parameters such as K m , V max , Apparent K cat , K cat / K m , and substrate (ATP) inhibition kinetic and substrate binding characteristics are significantly altered in the diabetic group. The observed changes are suggestive of compositional changes in the subunit makeup of HK and PFK. The implication of these findings in relation to energy status of the diabetic erythrocyte and its interrelationship with loss of cell deformability are discussed here.