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Structural insights into repression of the Pneumococcal fatty acid synthesis pathway by repressor FabT and co‐repressor acyl‐ACP
Author(s) -
Zuo Gang,
Chen ZhiPeng,
Jiang YongLiang,
Zhu Zhongliang,
Ding Chengtao,
Zhang Zhiyong,
Chen Yuxing,
Zhou CongZhao,
Li Qiong
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13534
Subject(s) - repressor , psychological repression , yy1 , biochemistry , dna , chemistry , fatty acid , gene , biology , promoter , gene expression
The Streptococcus pneumoniae fatty acid synthesis (FAS) pathway is globally controlled at the transcriptional level by the repressor FabT and its co‐repressor acyl carrier protein (acyl‐ACP), the intermediate of phospholipid synthesis. Here, we report the crystal structure of FabT complexed with a 23‐bp dsDNA, which indicates that FabT is a weak repressor with low DNA‐binding affinity in the absence of acyl‐ACP. Modification of ACP with a long‐chain fatty acid is necessary for the formation of a stable complex with FabT, mimicked in vitro by cross‐linking, which significantly elevates the DNA‐binding affinity of FabT. Altogether, we propose a putative working model of gene repression under the double control of FabT and acyl‐ACP, elucidating a distinct repression network for Pneumococcus to precisely coordinate FAS.