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Decrease in DNA methylation 1 interacts with chromomethylase and like heterochromatin protein 1 in Physcomitrella patens
Author(s) -
Parihar Vimala,
Dangwal Meenakshi,
Arya Deepshikha,
Kapoor Sanjay,
Kapoor Meenu
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13524
Subject(s) - heterochromatin , heterochromatin protein 1 , biology , dna methylation , methyltransferase , methylation , ezh2 , dna , genetics , dna methyltransferase , microbiology and biotechnology , chromatin , gene , gene expression
The nucleosome remodeling protein decrease in DNA methylation 1 (DDM1)/Lsh maintains normal levels of DNA methylation. Direct interaction between Lsh and DNA methyltransferase 1 (Dnmt1) and their localization to heterochromatin in the presence of heterochromatin protein‐1α (HP1α) is a mechanism by which the concentration of DNMTs is increased at heterochromatin, and chromosome structures are stabilized in metazoans. In plants, however, it is unclear how DDM1 cooperates with methyltransferases and like heterochromatin protein 1 (LHP1). In this study, we provide evidence for a novel interaction between moss DDM1 (PpDDM1) and the chromomethylase PpCMT, that has not been reported in any plant, and between PpDDM1 and PpLHP1, that has not been reported before in any organism. Our protein–protein interaction studies may provide mechanistic insight into heterochromatin regulation.