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Structure‐based control of the rate limitation of photosynthetic electron transport
Author(s) -
Ness Jillian,
Naurin Sejuti,
Effinger Katherine,
Stadnytskyi Valentyn,
Ibrahim Iskander M.,
Puthiyaveetil Sujith,
Cramer William A.
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13484
Subject(s) - electron transport chain , photosynthesis , chemistry , quinone , cytochrome , cytochrome c , electron transfer , biophysics , cytochrome f , biochemistry , photochemistry , photosystem ii , photosystem i , biology , enzyme , mitochondrion
The 2.5 Å structure of the cytochrome (cyt) b 6 f complex provides a basis for control of the rate‐limiting electron transfer step of oxygenic photosynthesis associated with the plastoquinol/quinone exchange pathway. Here, a structural change was made at a site containing two proline residues which border the intra‐cyt pathway for plastoquinol/quinone exchange. The proline side chains confer a larger aperture for passage of plastoquinol/quinone. Change of these prolines to alanine in the cyanobacterium Synechococcus sp. PCC 7002 results in attenuation of this rate‐limiting step, observed by a two‐fold reduction in the rate of cell growth, O 2 evolution, and plastoquinol‐mediated reduction of cyt f . This study demonstrates modification by site‐directed mutagenesis of photosynthetic energy transduction based on rational application of information in the atomic structure.