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Two‐step induction of cdsA promoters leads to upregulation of the glycolipid MPI ase at cold temperature
Author(s) -
Sawasato Katsuhiro,
Sekiya Yusei,
Nishiyama Kenichi
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13460
Subject(s) - downregulation and upregulation , chemistry , cold shock domain , glycolipid , microbiology and biotechnology , biochemistry , biology , rna , gene
Glycolipid MPI ase, essential for membrane protein integration into the cytoplasmic membrane of Escherichia coli , is upregulated at cold temperatures. This upregulation is rapid and sustainable. CdsA, a CDP ‐diacylglycerol synthase, is a rate‐limiting enzyme for MPI ase biosynthesis. Upregulation of CdsA is responsible for the increase in the MPI ase level at low temperature. Investigation of cdsA regulatory regions revealed at least two cold‐inducible promoters, a cold‐shock promoter that functions transiently and immediately in the cold, and one that is sustainable in the cold. The stability of the cdsA transcript was comparable with that of tufA , which is not cold‐inducible. Thus, cdsA is induced through two‐step cold‐induction to maintain MPI ase at a high level rapidly and sustainably in the cold.