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Metabolomic analysis of C2C12 myoblasts induced by the transcription factor FOXO 1
Author(s) -
Matsuda Rintaro,
Uchitomi Ran,
Oyabu Mamoru,
Hatazawa Yukino,
Kamei Yasutomi
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13445
Subject(s) - metabolite , glycolysis , c2c12 , transcription factor , biochemistry , metabolomics , foxo1 , chemistry , amino acid , enzyme , biology , microbiology and biotechnology , gene , myogenesis , chromatography
The transcription factor FOXO 1 is considered to play roles in the regulation of energy metabolism in various tissues. To determine the metabolic changes occurring due to FOXO 1 activation, we analyzed the metabolic profile of C2C12 myoblasts expressing a FOXO 1‐estrogen receptor fusion protein using capillary electrophoresis with electrospray ionization time‐of‐flight mass spectrometry ( CE ‐ TOFMS ). In FOXO 1‐activated cells, the metabolite levels during glycolysis are higher and the gene expression of pyruvate dehydrogenase kinase, an enzyme that inhibits glucose utilization, is increased. In addition, the metabolite levels of numerous amino acids are decreased, with increased gene expression of branched chain amino acid metabolism enzymes. Our results suggest that FOXO 1 suppresses glucose utilization and promotes the use of proteins/amino acids as energy sources in muscle cells, potentially during starvation.