Premium
A new mouse model for noninvasive fluorescence‐based monitoring of mitochondrial UCP 1 expression
Author(s) -
Kawarasaki Satoko,
Kuwata Hidetoshi,
Sawazaki Honami,
Sakamoto Tomoya,
Nitta Takahiro,
Kim ChuuSook,
Jheng HueiFen,
Takahashi Haruya,
Nomura Wataru,
Ara Takeshi,
Takahashi Nobuyuki,
Tomita Koichi,
Yu Rina,
Kawada Teruo,
Goto Tsuyoshi
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13430
Subject(s) - uncoupling protein , green fluorescent protein , transgene , brown adipose tissue , thermogenesis , microbiology and biotechnology , genetically modified mouse , thermogenin , mitochondrion , fluorescence , biology , chemistry , biochemistry , adipose tissue , gene , physics , quantum mechanics
Mitochondrial uncoupling protein 1 ( UCP 1) is well known for its thermogenic function in brown adipose tissue ( BAT ). Since UCP 1 expends energy on thermogenesis, UCP 1 activation has been considered an approach to ameliorate obesity. As a tool for uncovering yet unknown mechanisms of UCP 1 activation, we generated a transgenic mouse model in which UCP 1 expression levels are reflected in fluorescence derived from monomeric red fluorescent protein 1 ( mRFP 1). In these UCP 1‐ mRFP 1 BAC transgenic mice, fluorescence intensity mimics the change in UCP 1 expression levels evoked through physiological or pharmacological stimulation. This transgenic mouse model will be useful in the search for bioactive compounds with the ability to induce UCP 1 and for revealing undiscovered mechanisms of BAT activation.