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Identification and functional characterization of a bacterial homologue of Zeta toxin in Leishmania donovani
Author(s) -
Srivastava Akriti,
Garg Swati,
Jain Ravi,
Ayana Rajagopal,
Kaushik Himani,
Garg Lalit,
Pati Soumya,
Singh Shailja
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13429
Subject(s) - antitoxin , leishmania donovani , biology , toxin , leishmania , extracellular , programmed cell death , microbiology and biotechnology , biochemistry , apoptosis , immunology , leishmaniasis , visceral leishmaniasis , parasite hosting , world wide web , computer science
Zeta‐toxin is a cognate toxin of epsilon antitoxin of prokaryotic Type II toxin‐antitoxin system ( TA ) and play an important role in cell death. An orthologue of bacterial‐zeta‐toxin (BzT) was identified in Leishmania donovani with similar structural and functional features. Leishmania zeta‐toxin (named Ld_ζ1) harboring similar UNAG and ATP ‐binding pockets showed UNAG kinase and ATP ‐binding activity. An active Ld_ζ1 was found to express in infective extracellular promastigotes stage of L. donovani and episomal overexpression of an active Ld_ζ1domain‐triggered cell death. This study demonstrates the presence of prokaryotic‐like‐zeta‐toxin in eukaryotic parasite Leishmania and its association with cell death. Conceivably, phosphorylated UNAG or analogues, the biochemical mimics of zeta‐toxin function mediating cell death can act as a novel anti‐leishmanial chemotherapeutics.