Premium
Circ RNA wrap – a flexible pipeline for circ RNA identification, transcript prediction, and abundance estimation
Author(s) -
Li Lin,
Bu Dechao,
Zhao Yi
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13423
Subject(s) - rna , computational biology , identification (biology) , biology , microbiology and biotechnology , physics , gene , genetics , botany
Circular RNA (circ RNA ) is a recently described RNA molecule formed by backsplicing, with the 3′ and 5′ ends linked covalently. The study of circ RNA s using RNA ‐Seq data enables the analysis of genome‐wide endogenous circular transcripts. Although several algorithms for circ RNA analysis have been developed, they have thus far had limited application. Additionally, approaches for prediction and validation of the composition of exons in circ RNA s are still being developed. In this study, we measured the effectiveness of existing tools on collected and simulated data. Furthermore, we carried out a wide‐ranging study of circ RNA workflows, which we named circ RNA wrap. Application of circ RNA wrap on the included datasets reveals that our novel protocol can assist researchers in the study of circ RNA biology.