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A new Elf5 Cre ERT 2‐ GFP BAC transgenic mouse model for tracing Elf5 cell lineages in adult tissues
Author(s) -
Singh Snahlata,
Elenio Emily,
Leu Nicolae A.,
Romano RoseAnne,
Vaughan Andrew E.,
DeRiso Jennifer,
Surendran Kameswaran,
Chakrabarti Rumela
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13390
Subject(s) - haploinsufficiency , transcription factor , transgene , green fluorescent protein , genetically modified mouse , knockout mouse , in vivo , biology , microbiology and biotechnology , chemistry , gene , biochemistry , genetics , phenotype
Elf5 is a transcription factor known to regulate critical developmental processes and has been shown to act as a tumour suppressor in multiple cancers. Elf5 knockout mice are embryonically lethal, limiting in vivo studies pertaining to its function. Moreover, haploinsufficiency of Elf5 limits the use of current mouse models to investigate adult tissue distribution of Elf5. Here, we successfully generated Elf5 CreERT2‐GFP bacterial artificial chromosome ( BAC ) transgenic mice and show that Elf5 + cells are present in several adult tissues, where its expression was previously not known. Our study demonstrates the unique distribution of Elf5 + cells in multiple adult organs, which will facilitate future studies investigating the function of Elf5 in these tissues during homeostasis, repair and cancer.