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Why the c‐Fos/c‐Jun complex is extremely conserved: An in vitro evolution exploration by combining cDNA display and proximity ligation
Author(s) -
Kang ShouKai,
Chu XinYi,
Tian Tian,
Dong PengFei,
Chen BaiXue,
Zhang HongYu
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13388
Subject(s) - complementary dna , dna , transcriptional regulation , chemistry , biology , microbiology and biotechnology , genetics , gene , transcription factor
Transcriptional regulation involves a series of sophisticated protein–protein and protein– DNA interactions ( PPI and PDI ). Some transcriptional complexes, such as c‐Fos/c‐Jun and their binding DNA fragments, have been conserved over the past one billion years. Considering the thermodynamic principle for transcriptional complex formation, we hypothesized that the c‐Fos/c‐Jun complex may represent a thermodynamic summit in the evolutionary space. To test this, we invented a new method, termed One‐Pot‐seq, which combines cDNA display and proximity ligation to analyse PPI / PDI complexes simultaneously. We found that the wild‐type c‐Fos/c‐Jun complex is indeed the most thermodynamically stable relative to various mutants of c‐Fos/c‐Jun and binding DNA fragments. Our method also provides a universal approach to detect transcriptional complexes and explore transcriptional regulation mechanisms.