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N‐glycome inheritance from cells to extracellular vesicles in B16 melanomas
Author(s) -
Harada Yoichiro,
Kizuka Yasuhiko,
Tokoro Yuko,
Kondo Kiyotaka,
Yagi Hirokazu,
Kato Koichi,
Inoue Hiromasa,
Taniguchi Naoyuki,
Maruyama Ikuro
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13377
Subject(s) - glycome , glycosylation , glycan , melanoma , glycosyltransferase , chemistry , extracellular vesicles , asparagine , biochemistry , n linked glycosylation , extracellular , gene , microbiology and biotechnology , biology , glycoprotein , enzyme , cancer research
We investigated the correlation between metastatic behaviors of tumor cells and asparagine‐linked glycosylation (N‐glycosylation) of tumor‐derived extracellular vesicles ( EV s). Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N‐glycosylation. All melanoma variants and EV s have nearly identical profiles of de‐sialylated N‐glycans. The major de‐sialylated N‐glycan structures of cells and EV s are core‐fucosylated, tetra‐antennary N‐glycans with β1,6‐ N ‐acetylglucosamine branches. A few N‐glycans are extended by N ‐acetyllactosamine repeats. Sialylation of these N‐glycans may generate cell‐type‐specific N‐glycomes on EV s. Taken together, melanoma‐derived EV s show high expression of tumor‐associated N‐glycans, and the core structure profile is inherited during multiple selection cycles of B16 melanomas and from tumor cells to EV s.