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Ribosomal mutation in helix 32 of 18S rRNA alters fidelity of eukaryotic translation start site selection
Author(s) -
Antony A Charles,
Ram Anup Kumar,
Dutta Kalloly,
Alone Pankaj V.
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13369
Subject(s) - ribosomal rna , translation (biology) , 18s ribosomal rna , fidelity , eukaryotic translation , genetics , 23s ribosomal rna , ribosome , mutation , biology , selection (genetic algorithm) , computational biology , messenger rna , rna , gene , computer science , telecommunications , artificial intelligence
The 40S ribosome plays a critical role in start codon selection. To gain insights into the role of its 18S rRNA in start codon selection, a suppressor screen was performed that suppressed the preferential UUG start codon recognition (Suppressor of initiation codon: Sui − phenotype) associated with the eIF5 G31R mutant. The C1209U mutation in helix h32 of 18S rRNA was found to suppress the Sui − and Gcn − (failure to derepress GCN4 expression) phenotype of the eIF5 G31R mutant. The C1209U mutation suppressed Sui − and Gcd − (constitutive derepression of GCN4 expression) phenotype of eIF2β S264Y , eIF1 K60E , and eIF1A‐ΔC mutation. We propose that the C1209U mutation in 40S ribosomal may perturb the premature head rotation in ‘Closed/P IN ’ state and enhance the stringency of translation start site selection.