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Translation arrest as a protein quality control system for aberrant translation of the 3′‐UTR in mammalian cells
Author(s) -
Hashimoto Satoshi,
Nobuta Risa,
Izawa Toshiaki,
Inada Toshifumi
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13362
Subject(s) - translation (biology) , untranslated region , gene , three prime untranslated region , protein biosynthesis , biology , five prime untranslated region , stop codon , microbiology and biotechnology , in silico , genetics , messenger rna , computational biology
Read‐through or mutations of a stop codon resulting in translation of the 3′‐UTR produce potentially toxic C‐terminally extended proteins. However, quality control mechanisms for such proteins are poorly understood in mammalian cells. Here, a comprehensive analysis of the 3′‐UTRs of genes associated with hereditary diseases identified novel arrest‐inducing sequences in the 3′‐UTRs of 23 genes that can repress the levels of their protein products. In silico analysis revealed that the hydrophobicity of the polypeptides encoded in the 3′‐UTRs is correlated with arrest efficiency. These results provide new insight into quality control mechanisms mediated by 3′‐UTRs to prevent the production of C‐terminally extended cytotoxic proteins.