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Possible ATP trafficking by ATP ‐shuttles in the olfactory cilia and glucose transfer across the olfactory mucosa
Author(s) -
Acevedo Claudia,
Blanchard Kris,
Bacigalupo Juan,
Vergara Cecilia
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13346
Subject(s) - olfactory mucosa , cilium , microbiology and biotechnology , olfactory epithelium , olfactory system , chemistry , biology , biochemistry , neuroscience
Odor transduction in the cilia of olfactory sensory neurons involves several ATP ‐requiring enzymes. ATP is generated by glycolysis in the ciliary lumen, using glucose incorporated from surrounding mucus, and by oxidative phosphorylation in the dendrite. During prolonged stimulation, the cilia maintain ATP levels along their length, by unknown means. We used immunochemistry, RT ‐ PCR , and immunoblotting to explore possible underlying mechanisms. We found the ATP ‐shuttles, adenylate and creatine kinases, capable of equilibrating ATP . We also investigated how glucose delivered by blood vessels in the olfactory mucosa reaches the mucus. We detected, in sustentacular and Bowman's gland cells, the crucial enzyme in glucose secretion glucose‐6‐phosphatase, implicating both cell types as putative glucose pathways. We propose a model accounting for both processes.